Stability of Hor1-specific YAC-clones and physical mapping of Hor1-loci in barley |
| |
Authors: | W Michalek M Kleine H Dargatz G Wenzel A Jahoor |
| |
Institution: | Lehrstuhl für Pflanzenbau und Pflanzenzüchtung der TU München, D-85350 Freising, Germany, DE Institut für Pflanzenbau und Pflanzenzüchtung, Christian-Albrechts-Universit?t zu Kiel, Olshausenstrasse 40, D-24118 Kiel, Germany, DE Weissheimer Forschung, Abteilung Biotechnologie, Schaarstrasse 1, D-56626 Andernach, Germany, DE
|
| |
Abstract: | The hordeins are the major class of storage proteins in barley and are encoded by multigene families. Two YAC-clones specific
for the C-hordein-coding Hor1-locus of barley (Hordeum vulgare L.) were selected. The clones were constructed with DNA from the cultivars ‘Franka’ and ‘Hockey’ and have insert sizes of
330 kb and 350 kb, respectively. Performing partial digestions and hybridizations with vector-specific probes, a restriction
analysis was conducted using restriction enzymes with a 8-bp recognition sequence. Both clones cover the complete region of
the Hor1-locus, but exhibit a different pattern of restriction sites reflecting the polymorphic nature of the locus on the scale of
long-range restriction mapping. The maximal extent of the regions homologous to the Hor1-specific probe, pBSC5, was 105 kb in the ‘Hockey’-derived YAC and 190 kb in the yeast artificial chromosome constructed with
‘Franka’-DNA. Furthermore the high degree of instability observed with the Hor1-specific YAC-clones is discussed in conjunction with the structure of the Hor1-locus.
Received: 19 December 1996 / Accepted: 31 January 1997 |
| |
Keywords: | Yeast artificial chromosomes (YAC) Barley C-hordeins Physical mapping |
本文献已被 SpringerLink 等数据库收录! |
|