The use of the fluorescent probe 8-anilino-1-naphthalene sulphonic acid (ANS) as a histochemical stain in plant tissues |
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Authors: | P J GATES K J OPARKA |
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Institution: | University of Durham, Botany Department, Durham, DHl 3LE, U.K. |
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Abstract: | Abstract. The fluorescent probe 8-anilino-l-naphthalene sulphonic acid (ANS) has been evaluated as a histochemical stain for plant tissues. The wide specificity of the compound for hydrophobic binding sites limits its analytical use, but renders it of considerable value as a general fluorescent stain for use in epi-illumination fluorescence microscopy. Used in this way it is analogous to the light-microscope stain toluidine blue. ANS has also been found to be a sensitive vascular tracer. |
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Keywords: | 8-anilino-l-naphthalene sulphonic acid ANS fluorescence microscopy histochemical stain vascular tracer plant proteins |
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