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Polyethylene Glycol Distearate 600 With 10% 1-Hexadecanol; A Superior Embedding Wax for WARM Climates
Authors:Martin Sage
Institution:  a Marine Science Institute, The University of Texas, Port Aransas, Texas
Abstract:A mixture of 90% polyethylene glycol distearate 600 and 10% 1-hexadecanol has a melting point of approximately 43 C and is suitable as a replacement for conventional polyester wax where laboratory temperatures are above 24 C. Sections are attached to slides by floating out on 10% formalin and picked up on slides precoated with undiluted egg albumen. Since the wax is opaque, very small specimens can be embedded after fixation in a block of 0.5% agar before dehydration and embedding in wax, thus facilitating their handling and orientation. The wax mixture sections well with a razor blade in a holder.
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