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| 前蛋白转换酶Furin和PC7在小鼠母胎界面的动态表达及其在胚胎粘附和扩展中的作用 | |
| 引用本文： | 周智,张保华,祝诚,薛立群,王红梅.前蛋白转换酶Furin和PC7在小鼠母胎界面的动态表达及其在胚胎粘附和扩展中的作用[J].动物学报,2008,54(5). |
| 作者姓名： | 周智 张保华 祝诚 薛立群 王红梅 |
| 作者单位： | 1. 湖南农业大学动物科技学院,长沙,410128;中国科学院动物研究所,计划生育生殖生物学国家重点实验室,北京,100101 2. 中国科学院动物研究所,计划生育生殖生物学国家重点实验室,北京,100101;中国科学院研究生院,北京,100039 3. 中国科学院动物研究所,计划生育生殖生物学国家重点实验室,北京,100101 4. 湖南农业大学动物科技学院,长沙,410128 |
| 基金项目： | 国家自然科学基金，中国科学院院长基金，湖南省科技攻关项目 |
| 摘 要： | 通过免疫组化、免疫荧光和小鼠胚胎-子宫内膜上皮细胞共培养体系,研究了前蛋白转换酶Proprotein Convertases (PCs)家族中的Furin和PC7在小鼠妊娠早期子宫和胚胎中的表达及对胚胎植入的影响.结果显示:Furin和PC7在妊娠D1-D4小鼠子宫的腺上皮和D5-D7小鼠子宫的蜕膜、腺上皮高表达;PC7在植入前胚胎的2-细胞和4-细胞期表达很低,8-细胞期表达开始增加,囊胚期滋养外胚层有显著的高表达.Furin的抑制剂Dec-RVKR-CMK可显著抑制共培养体系中胚胎的粘附和扩展.以上结果表明,Furin在植入期胚胎的粘附和扩展中发挥重要作用.此外,Furin和PC7可能参与子宫内膜蜕膜化和早期胚胎发育. |
| 关 键 词： | 前蛋白转换酶 胚胎 子宫内膜 粘附 扩展 小鼠 |
Dynamic expression of Furin and PC7 at the maternal-fetal interface and their roles in adhesion and outgrowth of mouse embryos |
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| ZHOU Zhi,ZHANG Bao-Hua,ZHU Cheng,XUE Li-Qun,WANG Hong-Mei.Dynamic expression of Furin and PC7 at the maternal-fetal interface and their roles in adhesion and outgrowth of mouse embryos[J].Acta Zoologica Sinica,2008,54(5). | |
| Authors: | ZHOU Zhi ZHANG Bao-Hua ZHU Cheng XUE Li-Qun WANG Hong-Mei |
| Institution: | ZHOU Zhi1,2,ZHANG Bao-Hua2,3,ZHU Cheng2,XUE Li-Qun1,WANG Hong-Mei21.College of Animal Science , Technology,Hunan Agricultural University,Changsha 410128,China2.State Key Laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,Beijing 100101,China3.Graduate School of the Chinese Academy of Sciences,Beijing 100039,China |
| Abstract: | The expression and function of Furin and PC7 during mouse embryo implantation was investigated using immunohistochemistry,immunofluorescence and an in vitro embryo-endometrial epithelial cells co-culture system.Results showed that Furin and PC7 were mainly localized in the glandular epithelial cells from D1 to D4 of pregnancy and in decidual cells and glandular epithelium on days 5-7 of pregnancy.Expression of PC7 was hardly detectable in embryos at the 2-cell and 4-cell stages,but was significantly upregul... |
| Keywords: | Furin PC7 |
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