Spatially Resolved Top-Down Proteomics of Tissue Sections Based on a Microfluidic Nanodroplet Sample Preparation Platform |
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| Affiliation: | 1. Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, Washington, USA;2. Biological Sciences Division, Pacific Northwest National Laboratories, Richland, Washington, USA |
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| Abstract: | |
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| Keywords: | top-down proteomics nanoPOTS laser capture microdissection proteoform spatial proteomics quantitation ABC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0045" }," $$" :[{" #name" :" text" ," _" :" ammonium bicarbonate ACN" },{" #name" :" keyword" ," $" :{" id" :" kwrd0055" }," $$" :[{" #name" :" text" ," _" :" acetonitrile BUP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0065" }," $$" :[{" #name" :" text" ," _" :" bottom-up proteomics CV" },{" #name" :" keyword" ," $" :{" id" :" kwrd0075" }," $$" :[{" #name" :" text" ," _" :" compensation voltages DDM" },{" #name" :" keyword" ," $" :{" id" :" kwrd0085" }," $$" :[{" #name" :" text" ," _" :" n-dodecyl-beta-maltoside DMSO" },{" #name" :" keyword" ," $" :{" id" :" kwrd0095" }," $$" :[{" #name" :" text" ," _" :" dimethyl sulfoxide FA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0105" }," $$" :[{" #name" :" text" ," _" :" formic acid FAIMS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0115" }," $$" :[{" #name" :" text" ," _" :" field asymmetric ion mobility spectrometry HEK293" },{" #name" :" keyword" ," $" :{" id" :" kwrd0125" }," $$" :[{" #name" :" text" ," _" :" human embryonic kidney 293 cell line LCM" },{" #name" :" keyword" ," $" :{" id" :" kwrd0135" }," $$" :[{" #name" :" text" ," _" :" Laser Capture Microdissection LC-MS/MS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0145" }," $$" :[{" #name" :" text" ," _" :" liquid chromatography with tandem mass spectrometry MSI" },{" #name" :" keyword" ," $" :{" id" :" kwrd0155" }," $$" :[{" #name" :" text" ," _" :" mass spectrometry imaging nanoPOTS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0165" }," $$" :[{" #name" :" text" ," _" :" nanodroplet Processing in One pot for Trace Samples PfCs" },{" #name" :" keyword" ," $" :{" id" :" kwrd0175" }," $$" :[{" #name" :" text" ," _" :" proteoform clusters PP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0185" }," $$" :[{" #name" :" text" ," _" :" polypropylene PPI" },{" #name" :" keyword" ," $" :{" id" :" kwrd0195" }," $$" :[{" #name" :" text" ," _" :" protein–protein interaction ProMex" },{" #name" :" keyword" ," $" :{" id" :" kwrd0205" }," $$" :[{" #name" :" text" ," _" :" Protein Mass Extractor PrSMs" },{" #name" :" keyword" ," $" :{" id" :" kwrd0215" }," $$" :[{" #name" :" text" ," _" :" proteoform spectrum matches PTM" },{" #name" :" keyword" ," $" :{" id" :" kwrd0225" }," $$" :[{" #name" :" text" ," _" :" posttranslational modification RT" },{" #name" :" keyword" ," $" :{" id" :" kwrd0235" }," $$" :[{" #name" :" text" ," _" :" retention time STRING" },{" #name" :" keyword" ," $" :{" id" :" kwrd0245" }," $$" :[{" #name" :" text" ," _" :" Search Tool for the Retrieval of Interacting Gene/Proteins TCEP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0255" }," $$" :[{" #name" :" text" ," _" :" Tris(2-carboxyethyl)phosphine TDP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0265" }," $$" :[{" #name" :" text" ," _" :" top-down proteomics TFA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0275" }," $$" :[{" #name" :" text" ," _" :" tri-fluoroacetic acid TopFD" },{" #name" :" keyword" ," $" :{" id" :" kwrd0285" }," $$" :[{" #name" :" text" ," _" :" Top-down mass spectrometry feature detection TopPIC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0295" }," $$" :[{" #name" :" text" ," _" :" Top-down mass spectrometry-based Proteoform Identification and Characterization |
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