Identification of the Receptor-Binding Protein in Lytic Leuconostoc pseudomesenteroides Bacteriophages |
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| Authors: | Witold Kot Karin Hammer Horst Neve Finn K Vogensen |
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| Institution: | Department of Food Science, Faculty of Science, University of Copenhagen, Frederiksberg, Denmarka;Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark, Lyngby, Denmarkb;Department of Microbiology and Biotechnology, Max Rubner-Institut, Kiel, Germanyc |
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| Abstract: | Two phages, P793 and ΦLN04, sharing 80.1% nucleotide sequence identity but having different strains of Leuconostoc pseudomesenteroides as hosts, were selected for identification of the host determinant gene. Construction of chimeric phages leading to the expected switch in host range identified the host determinant genes as ORF21P793/ORF23ΦLN04. The genes are located in the tail structural module and have low sequence similarity at the distal end. |
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