Capture,Release, and Identification of Newly Synthesized Proteins for Improved Profiling of Functional Translatomes |
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| Affiliation: | 1. Department of Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, California, USA;2. Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco, San Francisco, California, USA |
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| Abstract: | |
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| Keywords: | nascent proteins translation O-propargyl-puromycin cleavable linker mTOR AHA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0020" }," $$" :[{" #name" :" text" ," _" :" L-azidohomoalanine BCA" },{" #name" :" keyword" ," $" :{" id" :" kwrd0030" }," $$" :[{" #name" :" text" ," _" :" bicinchoninic acid cRAP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0040" }," $$" :[{" #name" :" text" ," _" :" common Repository of Adventitious Proteins CRAPome" },{" #name" :" keyword" ," $" :{" id" :" kwrd0050" }," $$" :[{" #name" :" text" ," _" :" Contaminant Repository for Affinity Purification DMSO" },{" #name" :" keyword" ," $" :{" id" :" kwrd0060" }," $$" :[{" #name" :" text" ," _" :" dimethyl sulfoxide FC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0070" }," $$" :[{" #name" :" text" ," _" :" fold change GO" },{" #name" :" keyword" ," $" :{" id" :" kwrd0080" }," $$" :[{" #name" :" text" ," _" :" Gene Ontology HPG" },{" #name" :" keyword" ," $" :{" id" :" kwrd0090" }," $$" :[{" #name" :" text" ," _" :" L-homopropargylglycine LC-MS/MS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0100" }," $$" :[{" #name" :" text" ," _" :" liquid chromatography–tandem mass spectrometry mTOR" },{" #name" :" keyword" ," $" :{" id" :" kwrd0110" }," $$" :[{" #name" :" text" ," _" :" mammalian target of rapamycin MS" },{" #name" :" keyword" ," $" :{" id" :" kwrd0120" }," $$" :[{" #name" :" text" ," _" :" mass spectrometry OPP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0130" }," $$" :[{" #name" :" text" ," _" :" O-propargyl-puromycin OPP-ID" },{" #name" :" keyword" ," $" :{" id" :" kwrd0140" }," $$" :[{" #name" :" text" ," _" :" OPP-mediated identification OPP-ID using a cleavable biotin-azide linker RBP" },{" #name" :" keyword" ," $" :{" id" :" kwrd0160" }," $$" :[{" #name" :" text" ," _" :" RNA-binding protein SILAC" },{" #name" :" keyword" ," $" :{" id" :" kwrd0170" }," $$" :[{" #name" :" text" ," _" :" stable isotope labeling by amino acids in cell culture |
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