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| 考马斯亮蓝与牛血清白蛋白相互作用机理的研究 | |
| 引用本文： | CAO Wen-gen,曹稳根,赵海泉,焦庆才.考马斯亮蓝与牛血清白蛋白相互作用机理的研究[J].激光生物学报,2008,17(1):32-37. |
| 作者姓名： | CAO Wen-gen 曹稳根 赵海泉 焦庆才 |
| 作者单位： | 1. Department of Chemistry and Life Science,Suzhou College,Suzhou 234000,Anhui,China;College of Life Science,Anhui Agricultural University,Hefei 230036,Anhui,China 2. 宿州学院化学与生命科学系,安徽,宿州,234000;安徽农业大学生命科学学院,安徽,合肥,230036 3. 安徽农业大学生命科学学院,安徽,合肥,230036 4. 南京大学生命科学学院药物生物技术国家重点实验室,江苏,南京,210093 |
| 基金项目： | This work was supported by Natural Science Key Research Project of Anhui Provincial Education Department(KJ2007A131ZC)，Professor/Doctor Foundation of Suzhou College(2006jb05) |
| 摘 要： | 利用光谱探针技术研究在酸性溶液中考马斯亮蓝G-250(Coomassie brilliant blue G-250,CBBG)与牛血清白蛋白(bovine serum albumin,BSA)相互作用机理,考察了不同实验条件对CBBG-BSA复合物吸收光谱的影响。实验结果表明:CBBG与BSA相互作用产生光谱蓝移主要是由CBBG与BSA间的疏水相互作用引起,而静电作用则是形成CBBG-BSA蓝色复合物的必要条件。同时,CBBG聚集体的聚集程度是影响CBBG-BSA蓝色复合物形成的重要因素。 |
| 关 键 词： | 考马斯亮蓝 牛血清白蛋白 光谱探针 相互作用机理 |
| 文章编号： | 1007-7146（2008）01-0032-06 |
| 收稿时间： | 2007-10-12 |
| 修稿时间： | 2007年10月12 |
Mechanism of the Interaction between Coomassie Brilliant Blue and Bovine Serum Albumin |
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| CAO Wen-gen.Mechanism of the Interaction between Coomassie Brilliant Blue and Bovine Serum Albumin[J].ACTA Laser Biology Sinica,2008,17(1):32-37. | |
| Authors: | CAO Wen-gen |
| Abstract: | The mechanism of the interaction between Coomassie brilliant blue G-250(CBBG) and bovine serum albumin(BSA) was studied by spectra probe.The influence of different experimental conditions on the absorption spectra of CBBG-BSA mixtures was investigated.Through these experiments,one would draw the conclusion that the hypsochromic shift of the absorption spectra of CBBG-BSA reaction system results mainly from the hydrophobic interaction between CBBG and BSA,while the electrostatic interaction is still necessary for the formation of CBBG-BSA complex.Meanwhile,the aggregative degree of CBBG molecules plays an important role in influencing the formation of CBBG-BSA complex of the reaction system. |
| Keywords: | Coomassie brilliant blue G-250 bovine serum albumin spectra probe interaction mechanism |
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