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Evaluation of pentachlorophenol-degrading potentiality of Pseudomonas sp. in a soil microcosm
Authors:Gautam  Sumit Kumar  Sharma  Rajeev  Ahmad  AH  Thakur  Indu Shekhar
Institution:(1) Environmental Biotechnology Laboratory, Department of Environmental Sciences, College of Basic science and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar-, 236145, Uttaranchal, India;(2) Department of Pharmacology, College of Veterinary Science, G.B. Pant University of Agriculture and Technology, Pantnagar-, 263145, Uttaranchal, India
Abstract:Pseudomonas sp. strain IST103 obtained from a stable consortium was capable of degrading pentachlorophenol (PCP) as sole carbon and energy source. The PCP-degrading potentiality of the strain was determined by growth of bacteria in culture medium, utilization of PCP by high performance liquid chromatography (HPLC), chloride release and ring cleavage. The strain was applied in two set of soil microcosms containing 20 and 40% moisture, each having different concentrations, 0, 10, 100, 500, and 1000 mg/l, of PCP. The result showed significant utilization of PCP (77% in 45 days) and higher growth of bacterial strain when PCP was applied in 100 mg/l concentration at 40% moisture. Inhibitory effects on the growth of bacterial strain were seen in 500 and 1000 mg/l concentration.
Keywords:Bacterial consortium  chemostat  degradation  Pseudomonas sp    tetrachlorohydroquinone (TCH) and pentachlorophenol
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