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Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious shoot regeneration on leaf and stem segments |
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| Authors: | R. G. F. Visser E. Jacobsen A. Hesseling-Meinders M. J. Schans B. Witholt W. J. Feenstra |
| Affiliation: | (1) Department of Genetics, University of Groningen, Kerklaan 30, 9751 NN Haren, Netherlands;(2) Department of Biochemistry, University of Groningen, Nijenborgh 16, 9747 AG Groningen, Netherlands;(3) Present address: Department of Plant Breeding, Agricultural University, P.O. Box 386, 6700 AJ Wageningen, Netherlands |
| Abstract: | Transformed potato (Solanum tuberosum) plants were obtained from homozygous diploid potato by using a transformation procedure in combination with an adventitious shoot regeneration method. Leaf and stem explants were inoculated with an Agrobacterium tumefaciens strain which contained a binary vector (pVU 1011) carrying the neomycin phosphotransferase gene. Shoot regeneration most effectively on stem explants, occurred within six weeks directly from the explants without introducing a callus phase. A strong seasonal influence on transformation efficiencies was observed. Analysis of a number of randomly selected regenerated shoots for their ability to root and form shoots on kanamycin-containing medium shows that over 90% of the regenerated shoots obtained are transformed. In a number of shoots transformation was confirmed by a test for the presence and expression of the NPT-II gene. |
| Keywords: | Agrobacterium tumefaciens adventitious shoot regeneration transformation homozygous potato |
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