Reconstitution of Sperm Nuclei of Zebrafish (Danio rerio) in Xenopus Egg Extracts |
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Authors: | Jungo Imahara Mika Tokumoto Yoshitaka Nagahama Katsutoshi Ishikawa Toshinobu Tokumoto |
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Institution: | (1) Department of Biology and Geosciences, Faculty of Science, Shizuoka University, Shizuoka 422-8529, Japan, JP;(2) Laboratory of Reproductive Biology, National Institute for Basic Biology, Okazaki 444-8585, Japan, JP;(3) JSPS Research Fellow, JP |
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Abstract: | Cell-free extracts of Xenopus eggs cause cyclic change in permeabilized sperm nucleus, nuclear envelope breakdown, chromosome condensation, and reformation
of nuclei. In this study, the ability of cell-free extracts to cause similar changes in zebrafish sperm was examined. When
lysolecithin-treated sperm from zebrafish were incubated in Xenopus egg extracts, a series of changes in sperm nuclear morphology were observed periodically. These changes correlated with maturation-promoting
factor (MPF) activity. Furthermore, sperm nuclei of zebrafish replicated DNA during reconstitution in Xenopus egg extracts. These results showed that cell-free extracts of Xenopus egg possess the ability to cause cell-cycle-dependent changes in zebrafish sperm, implying the possibility of generating
transgenic zebrafish in a similar way to transgenic Xenopus.
Received October 21, 1999; accepted July 18, 2000. |
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Keywords: | : zebrafish reconstitution of sperm nucleus DNA synthesis MPF cell cycle |
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