Variations during leaf development of the relative amounts of two bean (Phaseolus vulgaris) chloroplast tRNAsPhe which differ in their minor nucleotide content |
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| Authors: | H Pfitzinger L Maréchal-Drouard D T N Pillay J H Weil P Guillemaut |
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| Institution: | (1) Institut de Biologie Moléculaire des Plantes du CNRS, Université Louis Pasteur, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France;(2) Department of Biology, University of Windsor, N9B 3P4, Ontario, Canada;(3) Institut Universitaire de Technologie Louis Pasteur, 3 rue de l'Argonne, 67000 Strasbourg, France |
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| Abstract: | Bean (Phaseolus vulgaris cv. Saxa) chloroplasts contain two tRNAPhe species, namely tRNAPhe1 and tRNAPhe2. By sequence determination, we show that tRNAPhe2 is identical to the previously sequenced tRNAPhe1 except for two undermodified nucleotides. By reversed-phase chromatography analyses, we demonstrate that the relative amounts of these two chloroplast tRNAsPhe vary during leaf development: in etiolated leaves the undermodified tRNAPhe2 only represents 15% of total chloroplast tRNAPhe, during development and greening it increases to reach 60% in 8-day-old leaves, and it then decreases to 9% in senescing leaves. |
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| Keywords: | bean chloroplast tRNAPhe leaf development nucleotides (minor) |
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