Immobilization of Candida rugosa lipase on sporopollenin from Lycopodium clavatum |
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| Authors: | Havva Tutar Elif Yilmaz Erol Pehlivan Mustafa Yilmaz |
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| Institution: | aDepartment of Chemistry, Selcuk University, Campus, 42031 Konya, Turkey;bDepartment of Chemical Engineering, Selcuk University, Campus, 42031 Konya, Turkey |
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| Abstract: | Sporopollenin is a natural polymer obtained from Lycopodium clavatum, which is highly stable with constant chemical structure and has high resistant capacity to chemical attack. In this study, immobilization of lipase from Candida rugosa (CRL) on sporopollenin by adsorption method is reported for the first time. Besides this, the enzyme adsorption capacity, activity and thermal stability of immobilized enzyme have also been investigated. It has been observed that under the optimum conditions (Spo-E(0.3)), the specific activity of the immobilized lipase on the sporopollenin by adsorption was 16.3 U/mg protein, which is 0.46 times less than that of the free lipase (35.6 U/mg protein). The pH and temperature of immobilized enzyme were optimized, which were 6.0 and 40 °C respectively. Kinetic parameters Vmax and Km were also determined for the immobilized lipase. It was observed that there is an increase of the Km value (7.54 mM) and a decrease of the Vmax value (145.0 U/mg-protein) comparing with that of the free lipase. |
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| Keywords: | Sporopollenin Physical adsorption Lycopodium clavatum Immobilization Lipase |
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