Antibacterial activity of a disaccharide isolated from Streptomyces sp. strain JJ45 against Xanthomonas sp. |
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Authors: | Yoon-Suk Kang Yukyung Lee Somi K Cho Keun-Hwa Lee Bum-Joon Kim Minkyun Kim Yoongho Lim & Moonjae Cho |
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Institution: | Department of Biochemistry, Cheju National University, Jeju, Korea;;Division of Bioscience and Biotechnology, BMIC, RCD, Konkuk University, Seoul, Korea;;Faculty of Biotechnology, College of Applied Life Sciences, Cheju National University, Jeju, Korea;;Department of Microbiology, School of Medicine, Cheju National University, Jeju, Korea;;Institute of Medical Science, Cheju National University, Jeju, Korea;;Department of Microbiology, Seoul National University School of Medicine, Seoul, Korea;and;Department of Agricultural Biotechnology, Center for Agricultural Biomaterials, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Korea |
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Abstract: | Of the 316 actinomycetes strains isolated from various habitats, Streptomyces sp. strain JJ45 showed the strongest antibiotic activity against the plant pathogenic bacteria Xanthomonas campestris pv. campestris and was thus chosen for further study. The 16S rRNA gene sequence (1500 bp) and rpoB gene partial sequence (306 bp) of Streptomyces strains JJ45A and JJ45B were determined. The respective strain JJ45B sequences exhibited 96.8% identity with the Streptococcus gelaticus 16S rRNA gene sequence and 98.4% identity with the Streptococcus vinaceus ATCC 27478 rpoB partial sequence. The fermentation broth of the JJ45B strain was extracted to find an inhibitor of bacterial growth. The distilled water extract showed the highest activity against pathogenic bacteria. The active molecule was isolated by column chromatography on polyacrylamide or silica gel, thin-layer chromatography, and HPLC. It showed growth inhibition activity only toward phytopathogenic Xanthomonas sp. The structure of the compound was identified as α- l -sorbofuranose (3→2)-β- d -altrofuranose based on the interpretation of the nuclear magnetic resonance spectra. |
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Keywords: | antibacterial activity Xanthomonas α-l-sorbofuranose (3→2)-β-d-altrofuranose |
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