Analytical techniques for cell fractions. XXI. Two-dimensional analysis of serum and tissue proteins: multiple isoelectric focusing

Two-dimensional electrophoresis, using isoelectric focusing in one dimension and electrophoresis in sodium dodecyl sulfate in the second, yields the highest resolution separation of proteins and protein subunits thus far obtained. For general research use, for genetic screening, and for clinical studies, it is important to be able to make these analyses easily, relatively rapidly, and reproducibly. This paper describes a device and methods for casting up to 20 isoelectric focusing gels in parallel, for running them in the same apparatus, and for recovering them in preparation for the second-dimension separations.