Identification of membrane-active regions of the HIV-1 envelope glycoprotein gp41 using a 15-mer gp41-peptide scan |
| |
Authors: | Moreno Miguel R Pascual Roberto Villalaín José |
| |
Affiliation: | Instituto de Biología Molecular y Celular, Universidad Miguel Hernández, E-03202 Elche-Alicante, Spain. |
| |
Abstract: | The identification of membrane-active regions of the ectodomain of the HIV-1 envelope glycoprotein gp41 has been made by determining the effect on membrane integrity of a 15-mer gp41-derived peptide library. By monitoring the effect of this peptide library on membrane leakage, we have identified three regions on the gp41 ectodomain with membrane-interacting capabilities: Region 1, which would roughly correspond to the polar sequence which follows the fusion domain and extends to the N-terminal heptad repeat region; Region 2, which would correspond to the immunodominant loop; and Region 3, which would correspond to the pre-transmembrane region of gp41. The identification of these three regions supports their direct role in membrane fusion as well as facilitating the future development of HIV-1 entry inhibitors. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|