FIRST REPORT OF THE CYANOTOXIN ANATOXIN‐A FROM APHANIZOMENON ISSATSCHENKOI (CYANOBACTERIA)1 |
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| Authors: | Susanna A Wood John Paul Rasmussen Patrick T Holland Rebecca Campbell Anna L M Crowe |
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| Institution: | 1. Cawthron Institute, Private Bag 2, Nelson 7001, New Zealand;2. 2Author for correspondence: e‐mail .;3. Cooperative Centre for Water Quality and Treatment, Australian Water Quality Centre, SA Water Corporation, Salisbury, SA, 5108, Australia;4. Australian Water Quality Centre, SA Water Corporation, Bolivar, SA, 5110, Australia;5. School of Pharmacy and Medical Sciences, Division of Health Sciences, University of South Australia, Adelaide, SA, 5001, Australia |
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| Abstract: | The taxonomy and toxicity of a single‐filament isolate from a filamentous cyanobacterial bloom in Lake Hakanoa (New Zealand) were examined by microscopy and liquid chromatography–mass spectrometry. Based on a morphological examination of environmental and cultured material, strain CAWBG02 was identified as Raphidiopsis mediterranea Skuja; however, subsequent phylogenetic analysis of the 16S rRNA gene sequence demonstrated that CAWBG02 was most likely to be a single culture of Aphanizomenon issatschenkoi (Usacev) Proshkina‐Lavrenko. Toxin testing confirmed that the original bloom and A. issatschenkoi isolate produced anatoxin‐a but did not produce homoanatoxin‐a or any cylindrospermopsins, saxitoxins, or microcystins. Despite the absence of cylindrospermopsin production, genes implicated in the biosynthesis of cylindrospermopsin were successfully amplified from A. issatschenkoi strain CAWBG02. To our knowledge, this is the first confirmation of an anatoxin‐a‐producing species in the Southern Hemisphere and the first report of anatoxin‐a production by A. issatschenkoi. |
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| Keywords: | anatoxin‐a Aphanizomenon issatschenkoi cyanobacteria cyanotoxin cylindrospermopsin New Zealand |
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