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| 利用毕赤酵母系统高效表达灵芝中的免疫调节因子LZ-8蛋白 | |
| 引用本文： | 宋细忠,肖建勇,巩伯梁,习梅兰,李刚.利用毕赤酵母系统高效表达灵芝中的免疫调节因子LZ-8蛋白[J].微生物学免疫学进展,2011,39(3):1-5. |
| 作者姓名： | 宋细忠 肖建勇 巩伯梁 习梅兰 李刚 |
| 作者单位： | 1. 江西百神药业股份有限公司,宜春,336000 2. 中山大学基因工程教育部重点实验室,广州,510275 3. 深圳科兴生物工程有限公司,深圳,518057 |
| 基金项目： | 广州市重大科技攻关项目 |
| 摘 要： | 根据Murasugi等发表的LZ-8基因的DNA序列,利用PCR方法从灵芝菌丝体的基因组DNA中扩增到lz-8基因.将该基因构建到毕赤酵母表达载体上,电激转化毕赤酵母.对转化子先后进行PCR和PCR-Southem鉴定,表明lz-8基因已转入毕赤酵母.转化子经发酵培养后用SDS-PAGE电泳方法检测发酵液,证明毕赤酵母... |
| 关 键 词： | LZ-8 免疫调节因子 毕赤酵母 高效表达 |
Highly efficient expression of a kind of immunregulator ( LZ-8 ) from Gannoderma lucidum in Pichia pastoris |
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| SONG Xi-zhong,XIAO Jian-yong,GONG Bo-liang,et al..Highly efficient expression of a kind of immunregulator ( LZ-8 ) from Gannoderma lucidum in Pichia pastoris[J].Progress In Microbiology and Immunology,2011,39(3):1-5. | |
| Authors: | SONG Xi-zhong XIAO Jian-yong GONG Bo-liang |
| Institution: | SONG Xi-zhong1,XIAO Jian-yong2,GONG Bo-liang3,et al.(1 Jiangxi Baishen Pharmaceutical Co.Ltd,Yichun 336000,China,2 Key Laboratory of gene engineering of the ministry of education,Sun Yat-sen University,Guangzhou 510275,3 Shenzhen Kexing Co.Ltd,Shenzhen,518057,China) |
| Abstract: | LZ-8 gene was amplified from genomic DNA of Ganoderma mycelia by PCR reaction according to its DNA sequence published by Murasugi et al,and then this gene was inserted into an expression vector and electroporated into Pichia pastoris.PCR and PCR-Southern results of transformants confirmed that LZ-8 gene had been transfered into P.pastoris.Fermentation solution of a transformant was examined by SDS-PAGE,and the result indicated that LZ-8 gene had been expressed in P.pastoris with a highest expression level o... |
| Keywords: | LZ-8 Immunomodulatory agent Pichia pastoris High efficient expression |
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