Prevalence of drug-resistant microbes in sepsis cases of catheter and fistula based haemodialysis |
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| Authors: | Rui Jiang Waqas Ahmed Habiba Daud Dawood Ahmed Salim Al-Rejaie Muhammad Awais Irshad Muhammad M Ijaz Khan Mohammed M Jalal Osama M Alshehri Mater H Mahnashi |
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| Institution: | 1. Department of Nephrology, Huzhou Central Hospital, Zhejiang, Huzhou 313003, PR China;2. Department of Microbiology, The University of Haripur, Haripur, Pakistan;3. Department of Medical Lab Technology, The University of Haripur, Haripur, Pakistan;4. Department of Pharmacology and Toxicology, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia;5. Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of Sialkot, Punjab, Pakistan;6. School of Chemical Engineering, Sungyunkwan University, Suwon, South Korea;7. Department of Mathematics and Statistics, Riphah International University I-14, Islamabad 44000, Pakistan;8. Department of Medical Laboratory Technology, Faculty of Applied Medical Sciences, University of Tabuk, Tabuk, SA, Saudi Arabia;9. Department of Clinical Laboratory Sciences, Collage of Applied Medical Science, Najran University, Najran, Saudi Arabia;10. Department of Pharmaceutical Chemistry, School of Pharmacy, Najran University, Najran, Saudi Arabia |
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| Abstract: | BackgroundChronic stage renal disease is a severe disease of the kidney which affects people globally. According to the global burden of diseases in 2010, this disease has caused more deaths worldwide and due to the high death rate, the ESRD (end-stage renal disease) is now ranked up from 27th to 18th range in the list.MethodologyDialysis samples were collected from the Haripur city and surrounding areas. Samples were inoculated on different selective media for bacterial growth. In addition, different biochemical tests were also performed for identification, where as the resistance genes were identified through a polymerase chain reaction.ResultOut of the total 100 dialysis patient’s blood samples, only 17 showed the presence of gram-positive bacteria i.e., Staphylococcus aureus while two shown the presence of gram-negative bacteria i.e., Klebsiella pneumoniaeee and Pseudomonas aeruginosa. While in molecular identification two antibiotic resistance genes muc and mecA belong to the staphylococcus strain shown their presence.ConclusionA high infection rate has been observed in fistula-based hemodialysis (17(77.27%)) as compares to catheter-based hemodialysis (5(22.3%) with no significant difference of incidence between the groups (p > 0.05). |
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| Keywords: | Drug resistance Sepsis Catheter Fistula Hemodialysis ESRD |
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