Somatic embryogenesis from leaf cultures of potato |
| |
Authors: | JayaSree T Pavan U Ramesh M Rao AV Jagan Mohan Reddy K Sadanandam A |
| |
Institution: | (1) Plant Biotechnology Research Unit, Department of Botany, Kakatiya University, Warangal, 506 009, A.P., India |
| |
Abstract: | An efficient procedure has been developed for inducing somatic embryogenesis from leaf cultures of potato cv. Jyothi. Leaf sections were initially cultured on 2,4-dichlorophenoxyacetic acid (2,4-D) + benzyladenine (BA) and -naphthaleneacetic acid (NAA) + BA supplemented Murashige and Skoog (MS) media. Nodular embryogenic callus developed from the cut ends of explants on media containing 2,4-D and BA, whereas compact callus developed on media containing NAA and BA. The explants with primary callus were subsequently moved onto MS media containing zeatin and/or gibberellic acid (GA3) and BA. Treatment with zeatin (22.8 M) and BA (10.0 M) resulted in the induction of the highest number of somatic embryos directly from meristematic centres produced on the nodular tissue. Embryo induction and maturation took place on this medium. The cotyledonary stage embryos developed into complete plantlets on hormone-free MS medium. A distinct feature of this study is the induction of somatic embryogenesis in leaf cultures of potato which has not been reported previously. |
| |
Keywords: | Solanum tuberosum cv Jyothi somatic embryogenesis zeatin |
本文献已被 SpringerLink 等数据库收录! |
|