腹腔注射法制备一氧化碳中毒迟发性脑病大鼠模型

目的:建立可靠的急性一氧化碳(CO)中毒迟发性脑病的动物模型。方法:雄性SD大鼠,分次腹腔注射CO染毒制备模型,动态监测尾血碳氧血红蛋白(HbCO)浓度;Morris水迷宫检测大鼠1-5w逃避潜伏期;尼氏染色及TUNEL原位末端凋亡染色检测大脑皮质及海马细胞损伤及凋亡。结果:染毒后,大鼠出现典型的CO重度中毒症状,体内血液HbCO浓度迅速升高,使用分次腹腔注射法,大鼠可维持长时间(>12h)高HbCO状态(HbCO>48%);中毒组大鼠水迷宫检测认知功能较对照组下降,病理学检查显示大鼠出现脑细胞损伤、凋亡明显。结论:本研究建立了一种较为符合迟发性脑病临床特征的动物模型,具有简单、可靠、重复性好的特点,为深入研究急性CO中毒致迟发性脑损伤的机制提供可靠基础。

Preparation of carbon monoxide poisoning delayed encephalopathyrat model by intraperitoneal injection

Objective:To establish a convenient and reliable delayed neuropsychologic sequelae rat model with acute carbon monoxide(CO) poisoning.Methods:Male SD rats were injected CO repeatedly,then dynamically monitored the carboxyhemoglobin(HbCO) level in blood for ascertaining the administration dose and process;Cognitive ability was measured by the escape latency of searching the objective in the Morris watermaze;Neuronal injury were detected by Nissl stain,TUNEL stain was used to assess apoptosis.Results:The HbCO...