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Nucleotide sequence and genomic structure analyses of the p70 subunit of the human Ku autoantigen: evidence for a family of genes encoding Ku (p70)-related polypeptides |
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| Authors: | Andrew J Griffith Joe Craft Janine Evans Tsuneyo Mimori John A Hardin |
| Institution: | (1) Department of Internal Medicine, Yale University School of Medicine, 333 Cedar Street, 06510 New Haven, CT, USA;(2) Department of Molecular Biophysics & Biochemistry, Yale University School of Medicine, 333 Cedar Street, 06510 New Haven, CT, USA;(3) Present address: Keio University School of Medicine, Tokyo, Japan;(4) Department of Internal Medicine, Medical College of Georgia, 1120 15th Street, 30912 Augusta, GA, USA |
| Abstract: | cDNA encoding the p70 polypeptide subunit of the human Ku autoantigen was isolated. In vitro expression analysis of the cDNA demonstrates that it encodes the entire open reading frame. Nucleotide sequence analysis and comparison to other previously described sequences indicate the existence of several single-nucleotide and amino acid polymorphisms. Southern blot analyses demonstrate the presence of multiple copies of homologous DNA sequences in the human genome. These data support the hypothesis that multiple genes encode a family of Ku(p70)-related polypeptides. |
| Keywords: | autoantibodies DNA-binding protein gene Ku protein recombinant DNA systemic lupus erythematosus |
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