Contrasting effect of dark-chilling on chloroplast structure and arrangement of chlorophyll-protein complexes in pea and tomato: plants with a different susceptibility to non-freezing temperature |
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| Authors: | Garstka Maciej Venema Jan Henk Rumak Izabela Gieczewska Katarzyna Rosiak Malgorzata Koziol-Lipinska Joanna Kierdaszuk Borys Vredenberg Wim J Mostowska Agnieszka |
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| Affiliation: | (1) Department of Metabolic Regulation, Institute of Biochemistry, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland;(2) Present address: Department of Biotechnology, Bioton Co. Ltd, 05-850 Ożarów Mazowiecki, Poland;(3) Department of Plant Anatomy and Cytology, Institute of Plant Experimental Biology, Faculty of Biology, University of Warsaw, 02-096 Warsaw, Poland;(4) Laboratory of Plant Physiology, University of Groningen, 9750 AA Haren, The Netherlands;(5) Department of Biophysics, Institute of Experimental Physics, Faculty of Physics, University of Warsaw, Zwirki i Wigury 93, 02-089 Warsaw, Poland;(6) Department of Plant Physiology, Wageningen University and Research Centre, 6703 BD Wageningen, The Netherlands |
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| Abstract: | The effect of dark-chilling and subsequent photoactivation on chloroplast structure and arrangements of chlorophyll–protein complexes in thylakoid membranes was studied in chilling-tolerant (CT) pea and in chilling-sensitive (CS) tomato. Dark-chilling did not influence chlorophyll content and Chl a/b ratio in thylakoids of both species. A decline of Chl a fluorescence intensity and an increase of the ratio of fluorescence intensities of PSI and PSII at 120 K was observed after dark-chilling in thylakoids isolated from tomato, but not from pea leaves. Chilling of pea leaves induced an increase of the relative contribution of LHCII and PSII fluorescence. A substantial decrease of the LHCII/PSII fluorescence accompanied by an increase of that from LHCI/PSI was observed in thylakoids from chilled tomato leaves; both were attenuated by photoactivation. Chlorophyll fluorescence of bright grana discs in chloroplasts from dark-chilled leaves, detected by confocal laser scanning microscopy, was more condensed in pea but significantly dispersed in tomato, compared with control samples. The chloroplast images from transmission-electron microscopy revealed that dark-chilling induced an increase of the degree of grana stacking only in pea chloroplasts. Analyses of O-J-D-I-P fluorescence induction curves in leaves of CS tomato before and after recovery from chilling indicate changes in electron transport rates at acceptor- and donor side of PS II and an increase in antenna size. In CT pea leaves these effects were absent, except for a small but irreversible effect on PSII activity and antenna size. Thus, the differences in chloroplast structure between CS and CT plants, induced by dark-chilling are a consequence of different thylakoid supercomplexes rearrangements. Dedicated to Prof. Zbigniew Kaniuga on the 25th anniversary of his initiation of studies on chilling-induced stress in plants. |
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| Keywords: | Chloroplast and thylakoid membrane structure Chilling-sensitive Chilling tolerant Chlorophyll– protein complexes Cation-induced thylakoid stacking O-J-D-I-P chlorophyll fluorescence Confocal laser scanning microscopy Dark-chilling stress Pea Tomato |
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