| 杆状病毒诱导昆虫细胞凋亡的初步研究 |
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| 引用本文： | 姚伦广,杜昌升,彭建新,洪华珠.杆状病毒诱导昆虫细胞凋亡的初步研究[J].微生物学报,2002,42(3):380-383,T002. |
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| 作者姓名： | 姚伦广 杜昌升 彭建新 洪华珠 |
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| 作者单位： | 华中师范大学昆虫学研究所,武汉,430079 |
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| 摘 要： | 细胞凋亡 (ａｐｏｐｔｏｓｉｓ)最早被定义为一种有秩序、受控制并按某种预定程序发展的生理性的自然死亡过程 (ｋｅｒｒｅｔａｌ1 972 ) ,其特征是细胞收缩 ,染色质凝聚成块状 ,形成凋亡小体1] 。但其最主要的特征是细胞ＤＮＡ受到一种被激活的内源性核酸酶的降解 ,凝胶电泳时呈现出特征性的梯形带 (Ｗｙｌｌｉｅ,1 980 )。病毒感染是常见导致细胞凋亡的重要因素 ,杆状病毒同样诱导昆虫细胞凋亡 ,同时 ,作为打破宿主防御体系的一种策略 ,杆状病毒可通过自身编码抗凋亡基因的表达 ,抑制细胞凋亡以利于自己的增殖2 ] 。细胞调亡和病毒抗凋…
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| 关 键 词： | 杆状病毒， 斜纹夜蛾细胞系， 细胞凋亡， 梯形带 |
| 文章编号： | 0001-6209(2002)03-0380-04 |
Preliminary Studies on the Apoptosis of Spodoptera litura Cells Induced by TWO Baculoviruses |
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| Yao Lunguang Du Changsheng Peng Jianxin Hong Huazhu.Preliminary Studies on the Apoptosis of Spodoptera litura Cells Induced by TWO Baculoviruses[J].Acta Microbiologica Sinica,2002,42(3):380-383,T002. |
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| Authors: | Yao Lunguang Du Changsheng Peng Jianxin Hong Huazhu |
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| Institution: | Institute of Entomology, Central China Normal University, Wuhan 430079, China. |
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| Abstract: | Microscopic examination of Spodoptera litura(SL-1) cell infected with AcMNPV or SfaMNPV revealed progressive cell blebbing starting at 8 h-12 h postinfection and culminating in total cell destruction at 24 h postinfection. The fragmentation of the infected cell nuclei was observed by stained with the specific fluorescent dye DAPI. Agarose gel electrophoresis analysis of the DNA extracted from infected cells show typical DNA ladder. All these supported that both viruses infected SL-1 cells undergo apoptosis. Through lipofectin transinfection, we observed that only the DNA of AcMNPV or SfaMNPV can also induce apopotosis of SL-1 cell. Virus titer analysis revealed that neither viruses can duplicate in SL-1 cells. |
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| Keywords: | Baculovirus Spodoptera litura cell Apopotosis DNA ladder |
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