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Galactosylated collagen matrix enhanced in vitro maturation of human embryonic stem cell-derived hepatocyte-like cells |
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| Authors: | Arefeh Ghodsizadeh Hossein Hosseinkhani Abbas Piryaei Behshad Pournasr Mostafa Najarasl Yosuke Hiraoka Hossein Baharvand |
| Affiliation: | 1. Department of Stem Cells and Developmental Biology at the Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, P.O. Box 19395-4644, Tehran, Iran 2. Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran 3. Centre of Excellence in Nanotechnology, Taipei Medical University, Taipei, 110, Taiwan 4. Graduate Institute of Biomedical Materials and Tissue Engineering, College of Oral Medicine, Taipei Medical University, Taipei, 110, Taiwan 5. Department of Biology and Anatomical Sciences, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran 6. Life Science Laboratory, Nitta Gelatin Inc., Osaka, 581-0024, Japan 7. Department of Developmental Biology, University of Science and Culture, ACECR, Tehran, Iran |
| Abstract: | Due to their important biomedical applications, functional human embryonic stem cell-derived hepatocyte-like cells (hESC-HLCs) are an attractive topic in the field of stem cell differentiation. Here, we have initially differentiated hESCs into functional hepatic endoderm (HE) and continued the differentiation by replating them onto galactosylated collagen (GC) and collagen matrices. The differentiation of hESC-HE cells into HLCs on GC substrate showed significant up-regulation of hepatic-specific genes such as ALB, HNF4α, CYP3A4, G6P, and ASGR1. There was more albumin secretion and urea synthesis, as well as more cytochrome p450 activity, in differentiated HLCs on GC compared to the collagen-coated substrate. These results suggested that GC substrate has the potential to be used for in vitro maturation of hESC-HLCs. |
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