HPLC测定7种龙胆科植物花中龙胆苦苷与獐牙菜苦苷的含量

目的:建立7种龙胆花中龙胆苦苷和獐牙菜苦苷含量测定的HPLC方法。方法:采用微波辅助动态回流法进行提取,色谱条件:Fusion-RP 80 A C18柱(150 mm×4.6 mm,5μm);流动相:甲醇-0.2%磷酸溶液梯度洗脱(0~25 min:15%~30%);流速:1 mL/min;柱温:30℃;检测波长240 nm。结果:7种龙胆花中獐牙菜苦苷和龙胆苦苷的色谱峰与共存组分完全达到基线分离,线性范围分别为0.105~0.945μg(r=0.999 9),0.3~0.7μg(r=0.999 9),平均加样回收率分别为97.8%(RSD=1.02%),98.9%(RSD=1.51%)。结论:所建立的方法测定快速,结果准确可靠。

Determination of Swertiamarin and Gentiopicroside in the Flowers of Seven Gentianaceae

Objective: To establish a HPLC method for the determination of swertiamarin and gentiopico-side in the flowers of seven genfianaceae. Methods: A new method-microwave - assisted reflux with magnetic stir extraction was used. Experiments were performed on Fusion - RP 80 A C_(18) ( 150 mm × 4.6 mm, 5μm) column , with the mobile phase consisting of methanol and 0. 2 % phosphoric acid at a flow rate of 1 mL/min. The operation was conducted in the gradient mode in which the ratio of methanol to 0.2% phosphoric acid varied from 15:85 to 30:70 in 2.5 min. The column temperature was 30 ℃ and the determination wavelength was 240 nm. Results: Chromatographic separation of swertiamarin, gentiop-icroside and the co-existed compounds achieved baseline resolution. The linear range were 0.105～0. 945 μg(r=1.999 9) and 0.3～0.7(r=0.999 9) separately, the average recovery were respectively 97.8% (RSD=1.02%) and 98.9% (RSD = 1.51%). Conclusion: The method is rapid and precise.