A microquantitative method to characterize lectin-induced cytoagglutination |
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| Authors: | E.M. Davis J.J. Starling E.F. Walborg |
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| Affiliation: | 1. The University of Texas Health Science Center at Houston, Graduate School of Biomedical Sciences, Houston, TX 77025 USA;2. The Department of Biochemistry, The University of Texas System Cancer Center, M. D. Anderson Hospital and Tumor Institute, Houston, TX 77025, USA |
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| Abstract: | A microquantitative method to characterize lectin-induced cytoagglutination reactions is described. The assay, which utilizes an electronic particle counter, measures the disappearance of single cells from cell suspensions containing various concentrations of lectin. The method is sensitive, requiring only μg quantities of lectin, reproducible, and amenable to thorough statistical analysis. It was also utilized to quantitate the inhibition of lectin-induced cytoagglutination by various saccharides. The method was employed to characterize ConA-induced agglutination of guinea pig erythrocytes and Novikoff ascites hepatoma cells, and to investigate several parameters which influence lectin-induced cytoagglutination reactions. |
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| Keywords: | To whom reprint requests should be addressed. |
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