Aptamers as functional nucleic acids:<Emphasis Type="Italic">In vitro</Emphasis> selection and biotechnological applications |
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Authors: | Kyung?Man?You Sang?Hyun?Lee Aesul?Im Email author" target="_blank">Sun?Bok?LeeEmail author |
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Institution: | (1) Division of Molecular and Life Sciences, Pohang University of Science and Technology, 790-784 Pohang, Korea;(2) Department of Chemical Engineering, Pohang University of Science and Technology, 790-784 Pohang, Korea |
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Abstract: | Aptamers are functional nucleic acids that can specially bind to proteins, peptides, amino acids, nucleotides, drugs, vitamins
and other organic and inorganic compounds. The aptamers are identified from random DNA or RNA libraries by a SELEX (systematic
evolution of ligands by exponential amplification) process. As aptamers have the advantage, and potential ability to be released
from the limitations of antibodies, they are attractive to a wide range of therapeutic and diagnostic applications. Aptamers,
with a high-affinity and specificity, could fulfil molecular the recognition needs of various fields in biotechnology. In
this work, we reviewed some aptamer selection techniques, properties, medical applications of their molecules and their biotechnological
applications, such as ELONA (enzyme linked oligonucleotide assay), flow cytometry, biosensors, electrophoresis, chromatography
and microarrays. |
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Keywords: | aptamer SELEX ELONA flow cytometry biosensor chromatography microarray |
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