用电压敏感染料光学记录膜电位

应用传统电生理方法如微电极和膜片钳技术，在记录较小的神经细胞和纤细的神经突起膜电位及同步记录神经细胞群的电活动等方面目前仍是一大难题。随着生理科学和神经生物学的发展，利用电压敏感染料光学记录膜电位技术已成为一种较为理想的新手段。本文对光学记录膜电位技术的发展史、染料特性和作用机制、光学成像及膜电位记录原理、目前的光学方法中某些不足及未来前景等做了较系统的介绍，并且简述了光学记录膜电位在电生理和神经生物学中的应用。

Optical Recording of Membrane Potential Using Voltage-Sensitive Dyes

Understanding the spatiotemporal features of the information processing occurring in any complex neural structure requires the monitoring and analysis of the activity in populations of neurons. Using voltage-sensitive dyes coupled with optical recording technique to monitor the electrical activity in populations of neurons or in multiple sites of a single neuron has become a promising approach. This approach is relatively ideal resolution because of non-invasive and high spatial and temporal resolution features. In this article, the principle of optical recording,its development history,the characteristic and mechanism of voltage-sensitive dyes as well as the difficulties and future of this new technique were reviewed. In addition, the application of optical method in electrophysiology and neurobiology was also discussed.