首页 | 本学科首页   官方微博 | 高级检索  
     


Alpha-oxo semicarbazone peptide or oligodeoxynucleotide microarrays
Authors:Olivier Christophe  Hot David  Huot Ludovic  Ollivier Nathalie  El-Mahdi Ouafâa  Gouyette Catherine  Huynh-Dinh Tam  Gras-Masse Hélène  Lemoine Yves  Melnyk Oleg
Affiliation:UMR CNRS 8525, Biological Institute of Lille, 1 rue du Pr Calmette 59021 Lille France.
Abstract:
We describe in this paper the preparation and characterization of semicarbazide glass slides and their use for the fabrication of microarrays using site-specific alpha-oxo semicarbazone ligation. The functional density and homogeneity of the semicarbazide glass slides were optimized by analyzing the reactivity of the layer toward a synthetic glyoxylyl fluorescent probe. Oligonucleotide microarrays were prepared by site-specific immobilization of glyoxylyl oligodeoxynucleotides. The slides were directly used in the hybridization assays using fluorescence detection and displayed a significant gain in sensibility as compared to the aldehyde glass slide/amino oligodeoxynucleotide chemistry. Semicarbazide slides were also used for the immobilization of a biotinylated peptide alpha-oxo aldehyde. The peptide microarrays allowed model interaction studies with streptavidin or an anti-biotin antibody.
Keywords:
本文献已被 PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号