正常小鼠肝和腹水型肝癌细胞核内酸溶性蛋白质磷酸化的比较研究

染色质的组成成分,组蛋白和非组蛋白在特异的蛋白激酶作用下可以发生磷酸化修饰,组蛋白和非组蛋白的磷酸化和脱磷酸化可能在染色质的结构,基因表达以及DNA复制中起着重要的作用。本文比较是小鼠腹水型肝癌细胞核和正常小鼠肝细胞核内酸溶性蛋白质及其磷酸化的差异。正常小鼠肝细胞核酸溶性蛋白质的电泳染色图谱有一条明显可见的组蛋白H_1~0蛋白带,而对小鼠腹水型肝癌来说,此带极浅,但在腹水型肝癌细胞核酸溶性蛋白质的电泳染色图谱上可见到表观分子量约为68K的一条蛋白带,而正常小鼠肝未见此带。此外,从电泳胶片~(32)P放射自显影图谱可见腹水型肝癌组蛋白H_1,H_2A和非组蛋白带Ⅱ(MW43K),带Ⅲ(MW.67K)带Ⅳ(M.w.97K)磷酸化程度明显高于正常小鼠肝。

THE PHOSPHORYLATION LEVEL OF NUCLEAR ACID SOLUBLE PROTEINS IN MOUSE ASCITIC HEPATOMA AND NORMAL LIVER

Histones and nonhistones are components of chromatin ,and can be modified by phosphorylation under the action of protein-kinase.Phosphorylation and dephos-phorylation of histones and nonhistones are important to the structure of chromatin,gene expression and DNA replication.In this paper,we report the differences found in the phosphorylation of the nuclear acid soluble proteins of normal mouse liver and those from ascitic hepatoma.Electrophorctic pattern of the proteins indicate that histone H10 is the only protein band in normal liver,but in ascitic hapatoma,another band (MW68K) is observed.In addition,autoradiograph pattern of 32p labeled nuclear proteins after electrophoresis showed that levels of phosphorylation of histone H1,H2A and nonhistones of band Ⅱ(MW43K)Ⅲ(MW66K) ,and Ⅳ (MW 97K) are higher in mouse ascitic hepatoma than there in normal mouse liver.