RETRACTED ARTICLE: Virus-induced gene silencing for functional analysis of selected genes |
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Authors: | Mandar R Godge Arunima Purkayastha Indranil Dasgupta Prakash P Kumar |
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Institution: | (1) Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, 117543, Singapore;(2) Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi, 110021, India |
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Abstract: | Virus-induced gene silencing (VIGS) is a technology that exploits an RNA-mediated antiviral defense mechanism and has been
shown to be of great potential in plant reverse genetics. Circumvention of plant transformation, methodological simplicity,
robustness, and speedy results makes VIGS an attractive alternative instrument in functional genomics, even in a high throughput
fashion. The system is well established in Nicotiana benthamiana, and efforts are being made to improve VIGS in other species, including monocots. Here, we discuss the issues specific to
the application of VIGS technology to determine gene function, which has revealed the roles of a variety of genes in disease
resistance, abiotic stress, cellular signaling and secondary metabolite biosynthesis.
M. R. Godge and A. Purkayastha made equal contributions and hence should be treated as joint first authors for this paper. |
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Keywords: | VIGS Viral vectors Gene silencing Functional genomics |
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