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Native transfer RNA catalyzes Diels-Alder reaction
Authors:Mielcarek Michal  Barciszewska Miroslawa Z  Sałanski Piotr  Stobiecki Maciej  Jurczak Janusz  Barciszewski Jan
Institution:Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12, 61704 Poznan, Poland.
Abstract:In this paper we show that transfer ribonucleic acids (tRNAs) catalyze the Diels-Alder cycloaddition reaction. A new DNA oxidative damage product, 6-furfuryladenine (kinetin) or its riboside (diene), was transformed with dimethyl acetylenedicarboxylate or maleic anhydride (dienophile). The reaction proceeds in the presence of tRNA at high pressure but not at ambient condition. If so tRNA in prebiotic conditions (RNA world) had at least two functions: catalytic and a carrier of genetic information. It means that tRNA at high pressure shows catalytic properties and is a true Diels-Alderase.
Keywords:RNA world  Diels-Alder reaction  RNA catalysis  Transfer RNA  High pressure  Kinetin
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