Commercially Available Antibodies to Human Tumour Necrosis Factor-α Tested for Cross-Reactivity with Ovine and Bovine Tumour Necrosis Factor-α using Flow Cytometric Assays |
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Authors: | J Dernfalk K Persson Waller A Johannisson |
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Institution: | (1) Department of Anatomy and Physiology, Swedish University of Agricultural Sciences, Uppsala, Sweden;(2) Department of Ruminant and Porcine Diseases, National Veterinary Institute, Uppsala, Sweden;(3) Department of Obstetrics and Gynaecology, Swedish University of Agricultural Sciences, Uppsala, Sweden; |
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Abstract: | A thorough understanding of the immune system, including the role of different cytokines, during inflammatory diseases in
ruminants could lead to the development of new diagnostic methods and treatments. Tumour necrosis factor-α (TNF-α) is an important
cytokine in the onset of the inflammatory responses. Unfortunately, the number of studies on cytokines, like TNF-α, in ruminants
is limited due to a lack of species-specific reagents. As cytokines have remained rather conserved during evolution, cross-reactivity
between animal species may occur. Therefore, the aim of the present study was to investigate 5 commercially available antibodies
against human TNF-α for their ability to cross-react with ovine and/or bovine TNF-α, using a bead-based flow cytometric method.
Two of the antibody clones (Mab 11 and 6401.1111) showed cross reactivity with ovine recombinant TNF-α in concentrations above
2.5 ng/ml. However, none of the antibodies detected TNF-α in bovine milk, or serum containing known concentrations of bovine
TNF-α, as earlier determined with ELISA. The results could be due to inability of the antibodies to cross-react between species,
but quenching of the signal by matrix proteins might also have lowered the response. |
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