Expression of plasma membrane proton-ATPase gene in salt-tolerant yeast Zygosaccharomyces rouxii is induced by sodium chloride |
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Authors: | Yasuo Watanabe Yumi Sanemitsu Youichi Tamai |
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Institution: | Laboratory of Biochemistry, Department of Biological Resources, Faculty of Agriculture, Ehime University, Matsuyama, Ehime 790, Japan |
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Abstract: | Abstract The amounts of mRNA and protein of plasma membrane proton-ATPase were measured in the salt-tolerant yeast Zygosaccharomyces rouxii by Northern and Western blot analyses. Although their amounts were independent of growth phase, their synthesis were induced when yeast cells were grown in the presence of NaCl or were subjected to NaCl shock. This finding was consistent with our previous result that plasma membrane proton-ATPase activity was elevated in Z. rouxii cells grown in medium containing high concentrations of NaCl. |
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Keywords: | Proton-ATPase Plasma membrane Salt-tolerance NaCl shock Zygosaccharomyces rouxii |
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