对虾白斑综合征病毒中国地方株变异区基因的比较

对虾白斑综合征病毒(White spot syndromevirus,WSSV)是对虾养殖的主要病原之一,它是目前发现的基因组最大的动物病毒,为环状双链DNA病毒1,2],全基因组序列分析结果显示,对虾白斑综合征病毒和其他杆状病毒相差甚远,最新病毒分类报告已将该病毒划归新建立的线头病毒科(Nima-viridae)白斑病毒属(Whispovirus)3,4]。目前Gen-Bank公布有3个版本的WSSV全序列1,2],其基因组大小的测定结果相差较大。不同的WSSV毒株可能在形态结构、理化性质上无法区分,但病毒基因组限制酶切片段长度多态性(RFLP)可以将之区分开来,Marks等6,7]通过计…

Comparison of Variable Region Genes of Shrimp White Spot Syndrome Virus (WSSV) in Different Areas in China

According to the conservative sequence in the epitaxial variable region of Thailand strain of WSSV published in GenBank,a pair of primers were designed to amplify the variable region genes of 5 local WSSV strains (ZHSH, ZHJ, HN, QD1, QD2) by PCR and then cloned. In accordance with the CN, the results indicated that the number of nucleotide of 5 strains were deleted more than 591bp of the TW and TH strains. The ZHSH and HN strains that deleted 591bp at the 3' end, and 454 bp at the 5' end of variable gene was highly homologous with CN strain about 99.3%. 229bp of ZHJ strain at the 5' end was homologous with CN about 99.3%, and deleted of 816bp at the 3' end. 97bp at the 5' end and 171bp at the 3' end of QD1 and QD2 strains were homologous with CN strain about 99.3%, and about 777bp were absent in the middle. The above data showed that the variable region genes of WSSV had mutated more in China. The variable region gene of QD2 strain was coincidence with that of QD1 after propagating 10 generations in crayfish. The results indicated that the crayfish inoculation did not result in mutation of variable region genes of WSSV.