Characterization and phylogenetic analysis of ectoine biosynthesis genes from <Emphasis Type="Italic">Bacillus halodurans</Emphasis> |
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| Authors: | Lawrance Anbu Rajan Toms C Joseph Nirmala Thampuran Roswin James Viswanathan Chinnusamy Kailash C Bansal |
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| Institution: | (1) Microbiology, Fermentation and Biotechnology Division, Central Institute of Fisheries Technology, Matsyapuri P.O., Cochin, 682029, Kerala, India;(2) Water Technology Centre, Indian Agricultural Research Institute, New Delhi, 110012, India;(3) National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110012, India |
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| Abstract: | Ectoine, a cyclic tetrahydropyrimidine (2-methyl-1,4,5,6-tetrahydropyrimidine-4-carboxylic acid), is a natural compound, which
serves as a protective substance in many bacterial cells. In this study, the putative ectABC gene cluster from Bacillus halodurans was heterologously expressed in E. coli and the production of ectoine was confirmed by HPLC analysis. The activity of the enzymes coded by the ectA, B and C genes were found to be higher in induced transgenic cells compared to the uninduced cells. Phylogenetic analysis revealed
sequence identities ranging from 36–73% for ectA gene, 55–81% for ectB gene and 55–80% for ectC gene indicating that the enzymes are evolutionarily well conserved. |
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| Keywords: | B halodurans Compatible solutes Salt stress Ectoine genes Osmoregulation |
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