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The differential effect of Ca-ATP and Mg-ATP on platelet actomyosin |
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| Authors: | J Abramowitz A Stracher T Detwiler |
| Affiliation: | 1. Department of Biosciences, University of Milan, via Celoria 26, 20133 Milano, Italy;2. Institute of Biophysics, National Research Council of Italy (CNR), 20133 Milano, Italy;1. Department of Clinical Pharmacology and Pharmacoepidemiology, University of Heidelberg, Im Neuenheimer Feld 410, 69120 Heidelberg, Germany;2. Institute of Experimental Physiology, Faculty of Biochemical and Pharmaceutical Science, Rosario National University, Suipacha 570, 2000 Rosario, Argentina |
| Abstract: | SDS-gel electrophoresis of the myosin peak produced by Mg-ATP splitting of platelet actomyosin on a 5–20% sucrose gradient revealed large amounts of actin or actin-like protein present. Substitution of Ca++ for Mg++ in the sucrose gradient released a lighter protein from the myosin peak. SDS-gel electrophoresis showed little actin or actin-like protein in the myosin peak and only actin or actin-like protein in the light peak. The differential effect of Ca-ATP and Mg - ATP on platelet actomyosin is not observed with skeletal muscle actomyosin. Futhermore the importance of calcium in platelet physiology suggests some important role for this Ca++-ATP sensitive actin or actin - like protein in platelet function. |
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