Cloning of the lacI gene into A ColE1 plasmid. |
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| Authors: | D L Hare J R Sadler |
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| Affiliation: | Department of Biophysics and Genetics, University of Colorado Medical Center, Denver, CO 80262, U.S.A. |
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| Abstract: | ![]()
The binding of lac repressor to lac operator was utilized to isolate an EcoRI fragment of lambda h80dlac (i+ as well as iq) that contains the lacI gene and lac promoter-operator regions. Ligation of this fragment into EcoRI cleaved pMB9 yielded chimeric DNA molecules of mol. w.t. 9.6 . 10(-6) and 1.5 . 10(7) daltons. Transformed strains containing the plasmids were analyzed for repressor production in vivo and in vitro. Repressor production in one plasmid strain is 7-fold greater than that in heat-inducible lambda h80dlac(iq)lysogens. |
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| Keywords: | Lactose repressor overproduction pMB9 plasmid BCIG IPTG |
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