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The basement membrane of hair follicle stem cells is a muscle cell niche |
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| Authors: | Fujiwara Hironobu Ferreira Manuela Donati Giacomo Marciano Denise K Linton James M Sato Yuya Hartner Andrea Sekiguchi Kiyotoshi Reichardt Louis F Watt Fiona M |
| Affiliation: | 1 Cancer Research UK Cambridge Research Institute, Li Ka Shing Centre, Robinson Way, Cambridge CB2 0RE, UK 2 Department of Medicine, Division of Nephrology, University of California San Francisco, San Francisco, CA 94158, USA 3 Department of Physiology, University of California San Francisco, San Francisco, CA 94158, USA 4 Fred Hutchinson Cancer Research Center, Seattle, WA 98109, USA 5 Institute for Protein Research, Osaka University, Osaka 565-0871, Japan 6 Department of Pediatrics and Adolescent Medicine, University of Erlangen-Nurnberg, 91054 Erlangen, Germany |
| Abstract: | The hair follicle bulge in the epidermis associates with the arrector pili muscle (APM) that is responsible for piloerection ("goosebumps"). We show that stem cells in the bulge deposit nephronectin into the underlying basement membrane, thus regulating the adhesion of mesenchymal cells expressing the nephronectin receptor, α8β1 integrin, to the bulge. Nephronectin induces α8 integrin-positive mesenchymal cells to upregulate smooth muscle markers. In nephronectin knockout mice, fewer arrector pili muscles form in the skin, and they attach to the follicle above the bulge, where there is compensatory upregulation of the nephronectin family member EGFL6. Deletion of α8 integrin also abolishes selective APM anchorage to the bulge. Nephronectin is a Wnt target; epidermal β-catenin activation upregulates epidermal nephronectin and dermal α8 integrin expression. Thus, bulge stem cells, via nephronectin expression, create a smooth muscle cell niche and act as tendon cells for the APM. Our results reveal a functional role for basement membrane heterogeneity in tissue patterning. PAPERCLIP: |
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