Conditions for open-air outdoor culture of Dunaliella salina in southern Spain |
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Authors: | M García-González J Moreno JP Cañavate V Anguis A Prieto C Manzano FJ Florencio MG Guerrero |
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Institution: | (1) Centro de Investigación y Cultivo de Especies Marinas "El Toruño", Apt. 16, Cádiz, 11500, Spain;(2) Consejo Superior de Investigaciones Científicas-Universidad de Sevilla, Instituto de Bioquímica Vegetal y Fotosíntesis, Avda. Américo Vespucio, s/n, Sevilla, 41092, Spain;(3) Consejería de Agricultura y Pesca. Junta de Andalucía, C/ Juan de Lara Nieto s/n, Sevilla, 41013, Spain |
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Abstract: | The optimization of the operation, under the climatic conditions of southern Spain, of an experimental plant for -carotene production by Dunaliella has been pursued. The effects of mixing, culture depth, cell density and dilution cycles on -carotene and biomass productivity were studied under a semicontinuous culture regime in open tanks outdoors. Using 3 m2-surface containers, the highest productivity values, for both -carotene and biomass, were recorded with a flow rate of 0.55 m s–1; 10 cm depth; 0.7 – 0.9 × 106cell ml–1, population density; and dilution cycles of two days. An average annual productivity of 1.65 g (dry wt) m–2 d–1 was estimated for Dunaliella biomass, being that for -carotene of about 0.1 g m–2 d–1. Under these optimized conditions, experiments have been carried out at the Cadiz Bay with 20 m2-surface tanks during a whole-year cycle. The results obtained have validated this location and the operating conditions established as being most appropriate for efficient mass production of -carotene rich D. salina. |
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Keywords: | -carotene" target="_blank">gif" alt="beta" align="MIDDLE" BORDER="0">-carotene Andalusian coast Biotechnology Dunaliella salina Microalga Outdoor culture |
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