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Identification of a novel compound that inhibits iNOS and COX-2 expression in LPS-stimulated macrophages from Schisandra chinensis |
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| Authors: | You Jin Lee Sun Young Park Sun Gun Kim Jum Soon Kang Sik Yoon Young Hun Kim Young-Whan Choi |
| Institution: | a Department of Horticultural Bioscience, Pusan National University, Miryang 627-706, Republic of Korea b Korea BIO-IT Foundry Center, Pusan National University, Busan 609-735, Republic of Korea c Department of Microbiology, Pusan National University, Busan 609-735, Republic of Korea d Department of Anatomy, School of Medicine, Pusan National University, Yangsan 626-770, Republic of Korea e Medical Research Center for Ischemic Tissue Regeneration, School of Medicine, Pusan National University, Yangsan 626-770, Republic of Korea f Department of Biochemistry, College of Medicine, Dong-A University, Busan 602-714, Republic of Korea |
| Abstract: | A novel α-iso-cubebenol, which has anti-inflammatory effects in lipopolysaccharide (LPS)-treated RAW 264.7 macrophages, was isolated from the fruits of Schisandra chinensis. α-iso-cubebenol inhibited LPS-induced nitric oxide (NO) and prostaglandin E2 (PGE2) production. Consistent with these findings, α-iso-cubebenol also reduced the LPS-induced expression of inducible nitric oxide synthase and cyclooxygenase-2 at the protein and mRNA levels in a concentration-dependent manner. α-iso-cubebenol also inhibited LPS-induced nuclear translocation of the NF-κB p65 subunit. Furthermore, α-iso-cubebenol suppressed the phosphorylation of ERK, JNK, and p38 kinase induced by LPS. Since the novel α-iso-cubebenol blocked the production of several pro-inflammatory mediators induced by LPS in macrophages, the molecule can be useful material for the development of anti-inflammatory agents against bacterial infections or endotoxin. |
| Keywords: | α-iso-cubebenol LPS iNOS COX-2 NF-κB Macrophage |
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