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Differentiating blood samples from scrapie infected and non-infected hamsters by detecting disease-associated prion proteins using Multimer Detection System |
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| Authors: | S.S.A. An K.T. Lim B.S. Lee Y.R. Ju S.Y. Kim |
| Affiliation: | a Department of Bionano Technology, Kyungwon University, Sungnam-si, Kyungki-do 461-701, South Korea b Department of Research and Development, PeopleBio Inc., Seoul, South Korea c Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, Szeged, Hungary d KNIH/CDC, Department of Neurovirology, Seoul, South Korea e National Institute of Animal Health, Prion Disease Research Center, Tsukuba, Japan f Seoul National University College of Medicine, Seoul National University Bundang Hospital Neuroscience Center, Bundang-gu, South Korea g Institute of Enzymology, Biological Research Center of the Hungarian Academy of Sciences, Budapest, Hungary h Department of Biochemistry and Molecular Biology, Faculty of Science and Informatics, University of Szeged, Szeged, Hungary |
| Abstract: | This communication describes the application of a modified sandwich enzyme-linked immunosorbent assay (ELISA), termed Multimer Detection System (MDS) for the detection of disease-associated multimeric forms of the prion protein (PrPd) in hamster blood. PrPd was detected in plasma of prion-affected hamsters while MDS revealed no PrPd in identically-treated plasma of healthy animals. This is the first report of a single ELISA- based immune detection of PrPd from blood samples. |
| Keywords: | TSE Prion Blood plasma Detection ELISA Aggregation Multimers Monomer Antibody |
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