|
|||||
|
|
Senescence-associated beta-galactosidase activity expression in aging hippocampal neurons |
|
| Authors: | Yi-Qun Geng |
| Institution: | a Central Laboratory, Joint Shantou International Eye Center of Shantou University and The Chinese University of Hong Kong, Shantou University Medica College, Dongxia Bei Road, Shantou, 515041 Guangdong, China b Department of Radiology, The Second Affiliated Hospital of Shantou, University Medical College, Dongxia Bei Road, Shantou, 515041 Guangdong, China c Department of Forensic Medicine, Shantou University Medical College, No. 22 Xinling Road, Shantou, 515041 Guangdong, China d Laboratory of Cell Senescence, Department of Etiology, Shantou University Medical College, No. 22 Xinling Road, Shantou, 515041 Guangdong, China |
| Abstract: | To investigate the activity of senescence-associated beta-galactosidase (SA-beta-GAL) in the hippocampus of aging rats. Hippocampi of 6-, 18-, and 24-month-old rats were observed by histochemical staining for SA-beta-GAL and cytochemical staining for SA-beta-GAL in cultured hippocampal neurons. The activity of SA-beta-GAL doubled in hippocampal pyramidal cells of the CA3 region in rats between 6 and 18 months (14.57 ± 2.74% vs. 31.66 ± 14.12% SA-beta-GAL-positive, respectively), and reached 50.76 ± 14.41% positive at 24 months. The activity of SA-beta-GAL also increased as a function of time upon prolonged culture of cultured hippocampal neurons with 95% of cells being SA-beta-GAL-positive at 20 days in vitro. Interestingly, no SA-beta-GAL-positive cells were found in neurons of the hippocampal dentate gyrus, a neurogenic region of the brain, at any age examined. SA-beta-GAL can be used as a senescence biomarker in determining senescent neurons in hippocampal pyramidal cells of the CA3 region in advanced aging. |
| Keywords: | Senescence-associated beta-galactosidase (SA-beta-GAL) Hippocampus Rat Biomarker |
| 本文献已被 ScienceDirect 等数据库收录！ | |