Isolation of gonadotrops from the pituitary of the African catfish,Clarias lazera |
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| Authors: | R de Leeuw H J Th Goos J Peute A M M van Pelt E Burzawa-Gérard P G W J van Oordt |
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| Institution: | (1) Zoological Laboratory, Research Group for Comparative Endocrinology, State University of Utrecht, The Netherlands;(2) Laboratoire de Physiologie Générale et Comparée, Paris, France;(3) Zoological Laboratory, State University of Utrecht, Padualaan 8, 3584 CH Utrecht, The Netherlands |
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| Abstract: | Summary Dispersed pituitary cells from male African catfish, Clarias lazera, were fractionated in a density gradient of Percoll. Five fractions were isolated, consisting of about 6, 19, 39, 95 and 83% gonadotrops, respectively. The gonadotrops were identified by their ultrastructural characteristics, by immunocytochemistry, and by measuring their hormone content. After one day in culture, in each fraction the secretion of gonadotropin could be stimulated by a luteinizing hormone-releasing hormone analogue, indicating that the cells had retained their functional integrity. Since the regulatory mechanisms of different cell types from the pituitary have some similarity, purification of the gonadotrops provides a model to study the regulation of gonadotropin secretion.A portion of the results was presented as a poster at the XIIth Conference of the European Society of Comparative Endocrinology, Sheffield, July 31–August 5, 1983 |
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| Keywords: | Gonadotrops Isolation Cell culture Gonadotropin secretion African catfish (Clarias lazera) |
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