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| rimJ基因缺失不影响大肠杆菌BL21(DE3)生长的温度敏感性 | |
| 引用本文： | 颛孙丹丹,方宏清,戴红梅,李树龙,谢迟,陈惠鹏.rimJ基因缺失不影响大肠杆菌BL21(DE3)生长的温度敏感性[J].生物技术通讯,2010,21(4):505-508. |
| 作者姓名： | 颛孙丹丹 方宏清 戴红梅 李树龙 谢迟 陈惠鹏 |
| 作者单位： | 1. 湖南农业大学,生物安全科学技术学院,湖南,长沙,410128 2. 军事医学科学院,生物工程研究所,北京,100071 3. 湖南师范大学,生命科学学院,湖南,长沙,410081 |
| 基金项目： | 国家自然科学基金面上项目 |
| 摘 要： | 目的:研究rimJ基因对大肠杆菌BL21(DE3)菌株生长的温度敏感性的影响。方法:利用SceⅠ-Red同源重组技术将大肠杆菌BL21(DE3)基因组中的rimJ基因缺失,得到rimJ缺失突变菌;比较缺失突变株和原始菌株在不同温度(25℃、37℃、42℃)下的最大比生长速率,利用统计学方法分析rimJ基因的缺失对BL21(DE3)菌株生长的温度敏感性是否有影响。结果:rimJ基因被成功敲除;统计分析结果表明缺失突变株和原始菌株的最大比生长速率没有明显区别。结论:rimJ基因缺失对大肠杆菌BL21(DE3)生长的温度敏感性无影响。 |
| 关 键 词： | rimJ基因 大肠杆菌BL21（DE3） 比生长速率 SceⅠ-Red同源重组 温度敏感性 |
Deletion of rimJ Gene Will Not Affect Temperature Sensitivity of Escherichia coli BL21(DE3) |
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| ZHUANSUN Dan-Dan,FANG Hong-Qing,DAI Hong-Mei,LI Shu-Long,XIE Chi,CHEN Hui-Peng.Deletion of rimJ Gene Will Not Affect Temperature Sensitivity of Escherichia coli BL21(DE3)[J].Letters in Biotechnology,2010,21(4):505-508. | |
| Authors: | ZHUANSUN Dan-Dan FANG Hong-Qing DAI Hong-Mei LI Shu-Long XIE Chi CHEN Hui-Peng |
| Institution: | 1. College of Bio-Safety Science and Technology, Hunan Agriculture University, Changsha 410128; 2. Beijing Institute of Biotechnology, Beijing 100071; 3. College of Biscience, Hunan Normal University, Changsha 410081; China |
| Abstract: | Objective： To study the effect of rimJ gene deletion on the temperature sensitivity of E.coli BL21 （DE3）. Methods： The SceⅠ-Red homologous recombination method was used to delete rimJ gene in E.coli BL21 （DE3） to generate the mutant strain. The maximum specific growth rates of wild-type and mutant strain at three different temperatures of 25℃, 37℃ and 42℃ were respectively measured and compared to analyze the temperature sensitivity difference between the two types of strains. Results： The PCR result showed that the rimJ gene was deleted as expected. There is no significant difference on the maximum specific growth rate between the wild type and the mutant according to the statistic analysis. Conclusion： Deletion of rimJ gene can not affect temperature sensitivity of E.coli BL21（DE3）. |
| Keywords: | rimJ gene E coli BL21（DE3） specific growth rate SceⅠ-Red homologous recombination temperature sensitivity |
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