A highly efficient ADH-coupled NADH-recycling system for the asymmetric bioreduction of carbon-carbon double bonds using enoate reductases |
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| Authors: | Tauber Katharina Hall Melanie Kroutil Wolfgang Fabian Walter M F Faber Kurt Glueck Silvia M |
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| Affiliation: | Department of Chemistry, Organic and Bioorganic Chemistry, University of Graz, Heinrichstrasse 28, A-8010 Graz, Austria. |
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| Abstract: | The asymmetric bioreduction of activated alkenes catalyzed by flavin-dependent enoate reductases from the OYE-family represents a powerful method for the production of optically active compounds. For its preparative-scale application, efficient and economic NADH-recycling is crucial. A novel enzyme-coupled NADH-recycling system is proposed based on the concurrent oxidation of a sacrificial sec-alcohol catalyzed by an alcohol dehydrogenase (ADH-A). Due to the highly favorable position of the equilibrium of ene-reduction versus alcohol-oxidation, the cosubstrate is only required in slight excess. |
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| Keywords: | cofactor recycling coupled‐enzyme approach alcohol dehydrogenase asymmetric bioreduction of alkene enoate reductase |
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