Cellular and Vacuolar Fusion of Protoplasts Electrofused Using Platinum Microelectrodes |
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Authors: | Morikawa, Hiromichi Hayashi, Yasuyuki Hirabayashi, Yasuzi Asada, Masanori Yamada, Yasuyuki |
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Affiliation: | Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University Kyoto 606, Japan |
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Abstract: | Protoplasts isolated from cultured cells of Coptis japonicaand Euphorbia millii were electrically fused using platinummicroelectrodes. The process involved two stages, cellular andvacuolar fusion, which are characterized respectively by transientwrinkling of the membrane and the formation of a dark-red precipitate. (Received June 12, 1987; Accepted October 13, 1987) |
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