The internal pH of Acetobacterium wieringae and Acetobacter aceti during growth and production of acetic acid |
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Authors: | Ulrike Menzel Gerhard Gottschalk |
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Institution: | (1) Institut für Mikrobiologie der Universität Göttingen, Grisebachstraße 8, D-3400 Göttingen, Federal Republic of Germany |
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Abstract: | The intracellular pH was measured in growing Acetobacterium wieringae and Acetobacter aceti with an acid equilibrium distribution method. 14C]-acetylsalicylic acid, 14C-benzoic acid and 14C]-acetic acid were used as pH-indicators. The extracellular pH of Acetobacterium wieringae decreased from 7.0 to 5.0 during growth; accordingly, the intracellular pH changed from 7.1 to 5.5, and a pH between 0.1 and 0.65 (interior more alkaline) was maintained. Corresponding results were obtained for Acetobacter aceti. The external pH and the internal pH decreased in parallel from 6.2 to 3.5 and from 5.8 to 3.9, respectively.This demonstrates that neither the anaerobic nor the aerobic acetogen was able to maintain a large pH in the presence of high concentrations of acetic acid. |
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Keywords: | Acetobacterium weiringae Acetobacter aceti Internal pH Acetic acid production |
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