L-valine production in Corynebacterium glutamicum based on systematic metabolic engineering: progress and prospects |
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Authors: | Liu Jie Xu Jian-Zhong Wang Bingbing Rao Zhi-Ming Zhang Wei-Guo |
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Institution: | 1.The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, 1800# Lihu Road, WuXi, 214122, People’s Republic of China ;2.National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, 1800# Lihu Road, WuXi, 214122, People’s Republic of China ; |
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Abstract: | L-valine is an essential branched-chain amino acid that cannot be synthesized by the human body and has a wide range of applications in food, medicine and feed. Market demand has stimulated people’s interest in the industrial production of L-valine. At present, the mutagenized or engineered Corynebacterium glutamicum is an effective microbial cell factory for producing L-valine. Because the biosynthetic pathway and metabolic network of L-valine are intricate and strictly regulated by a variety of key enzymes and genes, highly targeted metabolic engineering can no longer meet the demand for efficient biosynthesis of L-valine. In recent years, the development of omics technology has promoted the upgrading of traditional metabolic engineering to systematic metabolic engineering. This whole-cell-scale transformation strategy has become a productive method for developing L-valine producing strains. This review provides an overview of the biosynthesis and regulation mechanism of L-valine, and summarizes the current metabolic engineering techniques and strategies for constructing L-valine high-producing strains. Finally, the opinion of constructing a cell factory for efficiently biosynthesizing L-valine was proposed. |
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